| Product Name | PNC-27 Peptide Research/HDM2-binding Peptide PNC-27 |
| Amino Acid Sequence | PLSSQETFSDLWKLLKKWKKMRRNQFW VKVQRG (p53 residues 12-26 + penetratin) |
| NCI Thesaurus Code | C2934281 |
| MeSH Unique ID | C549751 |
| Storage | Store PNC-27 powder at -20°C; after reconstitution, aliquot and freeze immediately - avoid repeated freeze-thaw cycles |
Products Description
What Is PNC-27 Peptide?
PNC-27 (full name: HDM2-binding Peptide PNC-27) is an experimental anti-cancer peptide recognized by the National Cancer Institute (NCI) and the National Institutes of Health (NIH).
Applications and Research Uses of PNC-27 Peptide
Based on peer-reviewed studies from the National Institutes of Health (NIH) and the National Cancer Institute (NCI), here is a comprehensive overview of PNC-27 peptide applications.
1. Primary Application: Anti-Cancer Research
PNC-27's primary and most extensively studied application is as an experimental anti-cancer agent that induces tumor cell necrosis through a novel mechanism called "poptosis" (peptide-induced transmembrane pore formation
Mechanism-Based Application
The peptide works by binding to HDM-2 protein expressed on cancer cell membranes-a protein that is largely absent from normal cell membranes . This binding triggers:
| Step | Event | Outcome |
|---|---|---|
| 1 | PNC-27 forms 1:1 complexes with membrane-bound HDM-2 | Temperature-independent binding |
| 2 | Complexes dimerize to form transmembrane pores | Highly temperature-dependent |
| 3 | Cell contents rapidly leak out | Tumor cell necrosis (poptosis) |
2.Cancer Types Tested
PNC-27 has demonstrated cytotoxic activity against a wide range of human cancers :
Solid Tumors
| Cancer Type | Cell Lines Tested | Key Finding |
|---|---|---|
| Pancreatic Cancer | TUC-3, MIA-PaCa-2 | Complete cell killing; successful in vivo tumor eradication |
| Cervical Cancer | HTB-35 (SiHa) | IC50 = 12.4 μM |
| Breast Cancer | Multiple lines | Rapid total necrosis (within 1 hour) |
| Ovarian Cancer | Long-established lines | Effective on chemotherapy-resistant cells |
| Colon Cancer | Various lines | Cytotoxic |
| Non-Small Cell Lung Carcinoma | Various lines | Effective |
| Melanoma | A2058 | Effective in vitro and in vivo |
| Osteogenic Sarcoma | SAOS2 | Effective (p53-null) |
| Angiosarcoma | Various lines | Effective |
3.Diagnostic Application: HDM-2 Detection
The peptide's specific binding to HDM-2 has potential applications in detecting membrane-bound HDM-2 expression in:
1)Cervical cancer cells (stable in both cell culture and alcoholic preservative solutions) -
2)Rapidly growing tumors
3)Metastatic cancer cells
4.Research Applications: Mechanism Visualization
PNC-27 has been used as a tool to study transmembrane pore formation using high-resolution scanning immuno-electron microscopy, allowing direct visualization of pores in cancer cell membranes.
Benefits OF PNC-27 Peptide
Based on peer-reviewed studies from the National Institutes of Health (NIH) and the National Cancer Institute (NCI), here is a comprehensive overview of PNC-27 peptide's primary benefits in cancer research.
1.High Selectivity: Kills Cancer Cells, Spares Normal Cells
The most significant benefit of PNC-27 is its remarkable selectivity for cancer cells over normal cells.
| Cell Type | Sensitivity to PNC-27 | Reason |
| Cancer cells (solid & hematopoietic) | High | High HDM-2 expression on membrane |
| Normal cervical cells (PCS-480) | None | No membrane HDM-2 |
| Normal pancreatic acinar cells (BMRPA1) | None | No membrane HDM-2 |
| Normal hematopoietic stem cells | None | No membrane HDM-2 |
| Human fibroblasts, keratinocytes, HUVEC | None | No membrane HDM-2 |
Key finding from 2025 cervical cancer study: PNC-27 was cytotoxic to human cervical cancer cells (IC50 = 12.4 μM) but had no effect on normal cervical cells -2-5.
2024 AML research: PNC-27 selectively targets membrane HDM2 (mHDM2) expressed on AML blasts and leukemia stem cells (LSCs) but spares normal hematopoietic stem cells (HSCs).
2.Effective Against Chemotherapy-Resistant Cancers
A major advantage of PNC-27 is its ability to kill chemotherapy-resistant cancer cells. The anti-cancer effects do not depend on intracellular mechanisms that often confer drug resistance
Not dependent on:
1)Specific signal transduction pathways
2)DNA-repair processes
3)DNA methylation status
4)Multi-drug resistant (MDR) gene products
Evidence:
1)PNC-27 was cytotoxic to OVCAR-3 cells that are multi-drug-resistant
2)Effective on long-established chemotherapy-resistant human ovarian cancer cell lines
3)Kills primary ovarian cancer cells freshly isolated from patients
Important Disclaimer
All benefits described are from preclinical research studies (in vitro and animal models). PNC-27 is NOT an FDA-approved medical treatment. This information is for educational and research purposes only.
Customer Feedback
CONTACT PAGE
1.Inquiry methods：
Email:alice@xaheshun.com
Whatspp:+8618509260106
Online instant messaging
2.Contact Information：
Name：Alice
Telephone :+8618509260106
Email:alice@xaheshun.com
FAQ
Q: Does PNC-27 work on p53-deficient cancers?
A: Yes, this is a key advantage.
Q: What is the purity level of your PNC-27 peptide?
A: Our PNC-27 peptide is synthesized with a guaranteed purity ≥98%
Q: Is this PNC-27 peptide intended for human consumption or clinical use?
A: No. Our PNC-27 peptide is supplied strictly for laboratory research use and in vitro/in vivo experimental purposes only. It is not approved for human consumption, clinical trials, diagnostics, or therapeutic applications. All buyers must be qualified researchers or licensed institutions.
Q: What solvents are recommended for reconstituting PNC-27 peptide?
A: NC-27 is highly soluble in sterile water (H2O) or aqueous buffers like PBS at concentrations up to or exceeding 100 mg/mL. For cell culture work, we recommend reconstituting the stock solution in sterile water, filtering it through a 0.22μm filter, and then making final dilutions in the appropriate assay medium.
Q: What is the current research status of PNC-27?
A:According to the National Cancer Institute (NCI) , PNC-27 is classified as a "biologically active substance" in preclinical/translational research stage
Hot Tags: hdm2-binding peptide pnc-27, China hdm2-binding peptide pnc-27 manufacturers, suppliers, factory
